Bedekar, A.N.Pise, AbhinayNaik, AdeshBhosale, Paresh2012-01-202012-01-202012-01http://dspace.vpmthane.org:8080/jspui/handle/123456789/22404-Hydroxyisoleucine is reported as bioactive compound, which is responsible for antidiabetic activity of Fenugreek. Our present work Includes standardization and preparation of different extracts, which are enrich with 4- hydroxyisoleucine. The paper includes some unexpected observation in extraction pattern of 4-hydroxyisoleucine with different solvents. The quantitative estimation of 4-hydroxyisoleucine is done by spectrophotometry on the basis of its reaction with ninhydrin.en4-HydroxylsoleucineantidiabeticherbalextractionHPTLCspectrophotometryArticle