Standardisation of Fenugreek extract using 4-hydroxyisoleucine as a marker compound
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Date
2012-01
Journal Title
Journal ISSN
Volume Title
Publisher
J. Indian Chem. Soc
Abstract
4-Hydroxyisoleucine is reported as bioactive compound, which is responsible for
antidiabetic activity of Fenugreek. Our present work Includes standardization and preparation of
different extracts, which are enrich with 4- hydroxyisoleucine. The paper includes some unexpected
observation in extraction pattern of 4-hydroxyisoleucine with different solvents. The quantitative
estimation of 4-hydroxyisoleucine is done by spectrophotometry on the basis of its reaction with
ninhydrin.
Description
Keywords
4-Hydroxylsoleucine, antidiabetic, herbal, extraction, HPTLC, spectrophotometry