Separation and Properties of Rubisco and its Inhibitor Ca-1P from Solanum xanthocarpum Leaves
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Date
2012-03
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Publisher
Asian J. Research Chem. 5(2): February 2012
Abstract
Separation of Ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) with its naturally tightly bound inhibitor 2-
Carboxyarabinitol-1-phosphate (Ca-1P) from leaves of Solanum xanthocarpum was carried out using polyethylene
glycol precipitation method. The inhibitor was purified by ion exchange chromatography. The potential of Rubisco
activity was determined by the relative rate of biosynthesis and degradation. Rubisco had a Mass of 55 KDa with two
subunits, LSU (55 KD) and SSU (14 KD) showing single peak on HPLC. Properties of isolated CA1-P were analyzed
by TLC, HPLC, UV, IR and LC-MS. Rubisco efficiency may potentially lead to a faster plant growth, quicker
sequestration of CO2 from the air and more efficient of plant removal of green house gases from the atmosphere.
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Keywords
Photosynthesis, Rubisco, Ca-1P, Solanum xanthocarpum L, enzyme regulation, enzyme inhibitor