Standardisation of Fenugreek extract using 4-hydroxyisoleucine as a marker Compound

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    Standardisation of Fenugreek extract using 4-hydroxyisoleucine as a marker compound
    (J. Indian Chem. Soc, 2012-01) Bedekar, A.N.; Pise, Abhinay; Naik, Adesh; Bhosale, Paresh
    4-Hydroxyisoleucine is reported as bioactive compound, which is responsible for antidiabetic activity of Fenugreek. Our present work Includes standardization and preparation of different extracts, which are enrich with 4- hydroxyisoleucine. The paper includes some unexpected observation in extraction pattern of 4-hydroxyisoleucine with different solvents. The quantitative estimation of 4-hydroxyisoleucine is done by spectrophotometry on the basis of its reaction with ninhydrin.