Browsing by Author "Sawant, Neha"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Bioactive Molecule of Onion Leaves Act as Inhibitor of PPO.
    (Asian J. Research Chem, 2012-01) Goswami - Giri, Anita; Sawant, Neha
    Bioactive molecules of onion leaves exhibited potent anti polyphenol oxidase (PPO) activity; which was purified on silica gel by using solvents. Purification profile procured 3 and 2 active butanol and methanol fractions respectively. Reticence of PPO activity was observed within range of 12 to 14 min when it was treated with active fractions. Characterization of it was conceded by physical constant, UV, IR and TLC. It reputes that, active hydroxyl group attached to the ring structures of PPO, which quench free radical by donating hydrogen in the active hydroxyl group of PPO, confirming inhibition of pigmentation reaction in vitro. Bioactive molecules from onion leaves exhibited various types of inhibition against oxidation of L-DOPA and L-tyrosine and it was compared with classical inhibitors of PPO. Significance of bioactive molecules is to explore for drugs designing, understanding of ligand–receptor interactions, environmental problems and it is simple alternative for the inhibition of PPO.
  • Thumbnail Image
    Item
    Separation of Polyphenol Oxidase and its Inhibitor from Onion Leaves on Lignin Column
    (Asian Journal of Chemistry, 2012-01) Goswami - Giri, Anita; Sawant, Neha
    Tyrosine is a key compound of pigmentation/browning reaction through the action of polyphenol oxidase. Discrimination of this pigmentation reaction is responsible for various types of diseases and disorders. Core part of enzyme, polyphenol oxidase is same; therefore to regulate such discriminations of pigmentations/browning, searching for nontoxic endogenous inhibitor of this activity is important in developing the rational chemotherapy of pigmentation. Polyphenol oxidase and endogenous inhibitor was purified on natural matrix. Endogenous inhibitor eluted in two fractions which contains 2.15 ± 0.04 % of flavanoid and 6.1 mg/12.20 % of vitamin C. Fraction I (UV 245 nm, m.w. 66KDa) inhibits 100 % polyphenol oxidase at 14 min while fraction II (UV 224.5 nm, m.w. 98 KDa) inhibits polyphenol oxidase at 18 min. It was also confirmed by TLC.