Browsing by Author "Goswami - Giri, Anita"
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Item Bioactive Molecule of Onion Leaves Act as Inhibitor of PPO.(Asian J. Research Chem, 2012-01) Goswami - Giri, Anita; Sawant, NehaBioactive molecules of onion leaves exhibited potent anti polyphenol oxidase (PPO) activity; which was purified on silica gel by using solvents. Purification profile procured 3 and 2 active butanol and methanol fractions respectively. Reticence of PPO activity was observed within range of 12 to 14 min when it was treated with active fractions. Characterization of it was conceded by physical constant, UV, IR and TLC. It reputes that, active hydroxyl group attached to the ring structures of PPO, which quench free radical by donating hydrogen in the active hydroxyl group of PPO, confirming inhibition of pigmentation reaction in vitro. Bioactive molecules from onion leaves exhibited various types of inhibition against oxidation of L-DOPA and L-tyrosine and it was compared with classical inhibitors of PPO. Significance of bioactive molecules is to explore for drugs designing, understanding of ligand–receptor interactions, environmental problems and it is simple alternative for the inhibition of PPO.Item Composition and Characterization of Refined Oil Compared with Its Crude Oil from Waste Obtained from Mangifera indica(Asian J. Research Chem, 2012-01) Mahale, Saiprabha; Goswami - Giri, AnitaCrude and refined seed kernel oil was extracted from Alphanso mango aiming the study of physico-chemical properties. The oil produced was refined through degumming, neutralization and bleaching process using local adsorbent (activated clay). The specific gravity, refractive index, acid value, saponification value and iodine value for both crude and refined mango seed kernel oil was determined. The solid form refined mango kernel fat resembles cocoa butter when compared with physical and chemical characteristics. They both are thus, used as a substitute for each other or just as extenders. Mango Fat can be used as edible oil apart from its general uses in manufacturing soap, cosmetic formulations and industrial applications.Item Degradation of Solanum xanthocarpum Leaves Protein(Asian Journal of Chemistry, 2012-01) Pawar, Ashwini; Goswami - Giri, AnitaInside plant cells, the enzyme ribulose bisphosphate carboxylase/oxygenase (rubisco) forms the bridge between life and the lifeless, creating organic carbon from the inorganic carbon dioxide in the air. But only three carbon dioxide molecules are fixed by rubisco per second. Chloroplasts are filled with rubisco, which comprises half of the protein which contributes upto 50 % soluble protein and 30 % of total leaf nitrogen in leaves of C3 plants. This makes rubisco the most plentiful single enzyme on the earth. In the present study, Solanum xanthocarpum leaves protein and carbohydate contents was 12 and 18 %, respectively. It was used for the degradation and the neutralization of it for the nitrogen composition in the environment. Protein degradation considerd when 1 mM SDS and EDTA used under different stress conditions. Protein degradation limits at 60 ºC using tris buffer pH 8.0 at 1 h with stirring. Characterization and degree of dissociation of rubisco/protein was determined by splitting property of bands on gel electrophoresis and discrete spots on TLC.Item Separation and Properties of Rubisco and its Inhibitor Ca-1P from Solanum xanthocarpum Leaves(Asian J. Research Chem. 5(2): February 2012, 2012-03) Pawar, Ashwini; Goswami - Giri, AnitaSeparation of Ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) with its naturally tightly bound inhibitor 2- Carboxyarabinitol-1-phosphate (Ca-1P) from leaves of Solanum xanthocarpum was carried out using polyethylene glycol precipitation method. The inhibitor was purified by ion exchange chromatography. The potential of Rubisco activity was determined by the relative rate of biosynthesis and degradation. Rubisco had a Mass of 55 KDa with two subunits, LSU (55 KD) and SSU (14 KD) showing single peak on HPLC. Properties of isolated CA1-P were analyzed by TLC, HPLC, UV, IR and LC-MS. Rubisco efficiency may potentially lead to a faster plant growth, quicker sequestration of CO2 from the air and more efficient of plant removal of green house gases from the atmosphere.Item Separation of Polyphenol Oxidase and its Inhibitor from Onion Leaves on Lignin Column(Asian Journal of Chemistry, 2012-01) Goswami - Giri, Anita; Sawant, NehaTyrosine is a key compound of pigmentation/browning reaction through the action of polyphenol oxidase. Discrimination of this pigmentation reaction is responsible for various types of diseases and disorders. Core part of enzyme, polyphenol oxidase is same; therefore to regulate such discriminations of pigmentations/browning, searching for nontoxic endogenous inhibitor of this activity is important in developing the rational chemotherapy of pigmentation. Polyphenol oxidase and endogenous inhibitor was purified on natural matrix. Endogenous inhibitor eluted in two fractions which contains 2.15 ± 0.04 % of flavanoid and 6.1 mg/12.20 % of vitamin C. Fraction I (UV 245 nm, m.w. 66KDa) inhibits 100 % polyphenol oxidase at 14 min while fraction II (UV 224.5 nm, m.w. 98 KDa) inhibits polyphenol oxidase at 18 min. It was also confirmed by TLC.